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ATCC 101 l929 atcc cat
101 L929 Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/101+l929+atcc+cat/THP-1/pm38788712-864-124-126
Average 99 stars, based on 20139 article reviews

101 l929 atcc cat - by Bioz Stars, 2026-09

99/100 stars

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RNA Extraction:

Article Title: A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pTYB1 New England BioLabs Inc. Cat#N6701 pTYB1-FLAG-mTradd WT This Manuscript N/A pT7CFE1-CHis ThermoFisher 88860 T7CFE-mRipk3 This Manuscript N/A pcDNA3.1 Invitrogen Cat#V79520 pcDNA3-GFP-2xHA/2xStrep This Manuscript N/A pcDNA3.5-N2xHA-mRipk1 This Manuscript N/A pcDNA3-N3xHA-mZbp1 This Manuscript N/A pcDNA3-N3xHA-mRipk3 This Manuscript N/A pcDNA3-mTrif-C3xHA (Ticam1) This Manuscript N/A pcDNA3-N2xFLAG-mTradd This Manuscript N/A pLC-EGFP Gift from B. Bornhauser Addgene Cat# 75159 pLC-Cherry Gift from B. Bornhauser Addgene Cat# 75161 pSpCas9(BB)-2A-GFP Addgene 48138 pLC-EGFP-mTradd gRNA (CGC AACTGGACGATGAGCTG) This Manuscript N/A pLC-EGFP-mTnfr1 gRNA (CGG ACAGTCACTCACCAAGT) This Manuscript N/A pLC-Mlkl gRNA-CGM25 (CTGG CAGCAGGAAGATCGAC) This Manuscript N/A pLC-Ripk1 gRNA-CGM19 (AGG GAACTATTCGCTGGTGA) This Manuscript N/A pMA-hRipk1-gRNA (GCTC GGGCGCCATGTAGTAG) This Manuscript Synthetic, Invitrogen psPAX2 Addgene Cat# 12260 pMD2.G Addgene Cat# 12259 pBABE SV40 (D89-93) Gift from Parmjit Jat; Cotsiki et al.90 https://doi.org/10.1073/pnas.0308006100 Software and algorithms Dotmatics Dotmatics N/A Harmony High Content Analysis Software Perkin Elmer N/A ImageLab (v6.1.0) Bio-Rad N/A GraphPad Prism (v10.2.1) (339) Dotmatics N/A Adobe Illustrator (v28.0) (2024) Adobe N/A R studio package (v4.3.0) Limma N/A Fiji (v2.9.0) ImageJ N/A FlowJo (v.10) BD Biosciences N/A FACSDIVA software (v6.1.3) BD Biosciences N/A

Article Title: A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NaveniFlex MR Bethyl Laboratories Cat#NF.MR.100 CellTiter-GLO Promega Cat#G9681 RNeasy RNA extraction kit Qiagen Cat#74106 QuantiTec Reverse Transcription kit Qiagen Cat#205314 Pierce BCA protein assay kit Thermo Fisher Scientific Cat#23227 Deposited data Bulk RNA-seq (Sham Vs RT-treated EO771 cells) Sequence read archive ID: PRJNA1094427 Mass spectrometry: Human and mouse whole proteome analysis PRIDE ID: PXD043560 TCGA Pan-cancer analyses cBioPortal N/A SCAN-B TNBC patient data GEO Database GEO: GSE96058 GSEA analyses (hallmark gene set collection) The Molecular Signatures Database (MSigDB) N/A Raw western blots and image from this paper Mendeley https://doi.org/10.17632/822syk52yw.1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 HT1080 ATCC Cat#CCL-121 U937 ATCC Cat#CRL-3253 HT29 ATCC Cat#HTB-38 MDA-MB-231 ATCC Cat#CRM-HTB-26 MDA-MB-361 ATCC Cat#HTB-27 MDA-MB-468 ATCC Cat#HTB-132 HaCaT ATCC discontinued LLC ATCC Cat#CL-101 L929 ATCC Cat#CRL-6364 THP-1 ATCC Cat#TIB-202 EMT6 ATCC Cat#CRL-2755 Lim1215 Laboratory of Prof. Chris Marshall N/A A431 ATCC Cat#CRL-1555 F3II Laboratory of Prof. Daniel Alonso N/A D2A1 Laboratory of Prof. Clare Isacke N/A Kym-1 Laboratory of Prof. John Silke N/A Immortalized MDFs Charles River (Cells generated in house) N/A MCA-205 Sigma Aldrich Cat#SCC-173 EO771 CH3 Biosystems Cat#94A001 MC38 Kerafast Cat#ENH204-FP Wildtype BMDMs and Lung fibroblasts (C57BL/6) Charles River (Cells generated in house) N/A Spata2-/- BMDMs (C57BL/6) Laboratory of Prof. Mads Gyrd-Hansen’s N/A Trif-/- BMDMs (C57BL/6) (Ticam1-/-) Laboratory of Prof. Manolis Pasparakis N/A Zbp1-/- BMDMs (C57BL/6) Laboratory of Prof. Manolis Pasparakis N/A Trif-/-Zbp1-/- BMDMs and LFs (C57BL/6) (Ticam1-/-Zbp1-/-) Laboratory of Prof. Manolis Pasparakis N/A L929 Tradd-/- This Manuscript N/A L929 Tradd-/- reconstituted with TRADDWT This Manuscript N/A L929 Tradd-/- reconstituted with TRADD DD-only This Manuscript N/A L929 Tradd-/- reconstituted with TRADD Y16A/F18A This Manuscript N/A (Continued on next page) e5 Immunity 57, 1–19.e1–e15, July 9, 2024

Reverse Transcription:

Article Title: A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NaveniFlex MR Bethyl Laboratories Cat#NF.MR.100 CellTiter-GLO Promega Cat#G9681 RNeasy RNA extraction kit Qiagen Cat#74106 QuantiTec Reverse Transcription kit Qiagen Cat#205314 Pierce BCA protein assay kit Thermo Fisher Scientific Cat#23227 Deposited data Bulk RNA-seq (Sham Vs RT-treated EO771 cells) Sequence read archive ID: PRJNA1094427 Mass spectrometry: Human and mouse whole proteome analysis PRIDE ID: PXD043560 TCGA Pan-cancer analyses cBioPortal N/A SCAN-B TNBC patient data GEO Database GEO: GSE96058 GSEA analyses (hallmark gene set collection) The Molecular Signatures Database (MSigDB) N/A Raw western blots and image from this paper Mendeley https://doi.org/10.17632/822syk52yw.1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 HT1080 ATCC Cat#CCL-121 U937 ATCC Cat#CRL-3253 HT29 ATCC Cat#HTB-38 MDA-MB-231 ATCC Cat#CRM-HTB-26 MDA-MB-361 ATCC Cat#HTB-27 MDA-MB-468 ATCC Cat#HTB-132 HaCaT ATCC discontinued LLC ATCC Cat#CL-101 L929 ATCC Cat#CRL-6364 THP-1 ATCC Cat#TIB-202 EMT6 ATCC Cat#CRL-2755 Lim1215 Laboratory of Prof. Chris Marshall N/A A431 ATCC Cat#CRL-1555 F3II Laboratory of Prof. Daniel Alonso N/A D2A1 Laboratory of Prof. Clare Isacke N/A Kym-1 Laboratory of Prof. John Silke N/A Immortalized MDFs Charles River (Cells generated in house) N/A MCA-205 Sigma Aldrich Cat#SCC-173 EO771 CH3 Biosystems Cat#94A001 MC38 Kerafast Cat#ENH204-FP Wildtype BMDMs and Lung fibroblasts (C57BL/6) Charles River (Cells generated in house) N/A Spata2-/- BMDMs (C57BL/6) Laboratory of Prof. Mads Gyrd-Hansen’s N/A Trif-/- BMDMs (C57BL/6) (Ticam1-/-) Laboratory of Prof. Manolis Pasparakis N/A Zbp1-/- BMDMs (C57BL/6) Laboratory of Prof. Manolis Pasparakis N/A Trif-/-Zbp1-/- BMDMs and LFs (C57BL/6) (Ticam1-/-Zbp1-/-) Laboratory of Prof. Manolis Pasparakis N/A L929 Tradd-/- This Manuscript N/A L929 Tradd-/- reconstituted with TRADDWT This Manuscript N/A L929 Tradd-/- reconstituted with TRADD DD-only This Manuscript N/A L929 Tradd-/- reconstituted with TRADD Y16A/F18A This Manuscript N/A (Continued on next page) e5 Immunity 57, 1–19.e1–e15, July 9, 2024

Bicinchoninic Acid Protein Assay:

Article Title: A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NaveniFlex MR Bethyl Laboratories Cat#NF.MR.100 CellTiter-GLO Promega Cat#G9681 RNeasy RNA extraction kit Qiagen Cat#74106 QuantiTec Reverse Transcription kit Qiagen Cat#205314 Pierce BCA protein assay kit Thermo Fisher Scientific Cat#23227 Deposited data Bulk RNA-seq (Sham Vs RT-treated EO771 cells) Sequence read archive ID: PRJNA1094427 Mass spectrometry: Human and mouse whole proteome analysis PRIDE ID: PXD043560 TCGA Pan-cancer analyses cBioPortal N/A SCAN-B TNBC patient data GEO Database GEO: GSE96058 GSEA analyses (hallmark gene set collection) The Molecular Signatures Database (MSigDB) N/A Raw western blots and image from this paper Mendeley https://doi.org/10.17632/822syk52yw.1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 HT1080 ATCC Cat#CCL-121 U937 ATCC Cat#CRL-3253 HT29 ATCC Cat#HTB-38 MDA-MB-231 ATCC Cat#CRM-HTB-26 MDA-MB-361 ATCC Cat#HTB-27 MDA-MB-468 ATCC Cat#HTB-132 HaCaT ATCC discontinued LLC ATCC Cat#CL-101 L929 ATCC Cat#CRL-6364 THP-1 ATCC Cat#TIB-202 EMT6 ATCC Cat#CRL-2755 Lim1215 Laboratory of Prof. Chris Marshall N/A A431 ATCC Cat#CRL-1555 F3II Laboratory of Prof. Daniel Alonso N/A D2A1 Laboratory of Prof. Clare Isacke N/A Kym-1 Laboratory of Prof. John Silke N/A Immortalized MDFs Charles River (Cells generated in house) N/A MCA-205 Sigma Aldrich Cat#SCC-173 EO771 CH3 Biosystems Cat#94A001 MC38 Kerafast Cat#ENH204-FP Wildtype BMDMs and Lung fibroblasts (C57BL/6) Charles River (Cells generated in house) N/A Spata2-/- BMDMs (C57BL/6) Laboratory of Prof. Mads Gyrd-Hansen’s N/A Trif-/- BMDMs (C57BL/6) (Ticam1-/-) Laboratory of Prof. Manolis Pasparakis N/A Zbp1-/- BMDMs (C57BL/6) Laboratory of Prof. Manolis Pasparakis N/A Trif-/-Zbp1-/- BMDMs and LFs (C57BL/6) (Ticam1-/-Zbp1-/-) Laboratory of Prof. Manolis Pasparakis N/A L929 Tradd-/- This Manuscript N/A L929 Tradd-/- reconstituted with TRADDWT This Manuscript N/A L929 Tradd-/- reconstituted with TRADD DD-only This Manuscript N/A L929 Tradd-/- reconstituted with TRADD Y16A/F18A This Manuscript N/A (Continued on next page) e5 Immunity 57, 1–19.e1–e15, July 9, 2024

RNA Sequencing:

Article Title: A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NaveniFlex MR Bethyl Laboratories Cat#NF.MR.100 CellTiter-GLO Promega Cat#G9681 RNeasy RNA extraction kit Qiagen Cat#74106 QuantiTec Reverse Transcription kit Qiagen Cat#205314 Pierce BCA protein assay kit Thermo Fisher Scientific Cat#23227 Deposited data Bulk RNA-seq (Sham Vs RT-treated EO771 cells) Sequence read archive ID: PRJNA1094427 Mass spectrometry: Human and mouse whole proteome analysis PRIDE ID: PXD043560 TCGA Pan-cancer analyses cBioPortal N/A SCAN-B TNBC patient data GEO Database GEO: GSE96058 GSEA analyses (hallmark gene set collection) The Molecular Signatures Database (MSigDB) N/A Raw western blots and image from this paper Mendeley https://doi.org/10.17632/822syk52yw.1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 HT1080 ATCC Cat#CCL-121 U937 ATCC Cat#CRL-3253 HT29 ATCC Cat#HTB-38 MDA-MB-231 ATCC Cat#CRM-HTB-26 MDA-MB-361 ATCC Cat#HTB-27 MDA-MB-468 ATCC Cat#HTB-132 HaCaT ATCC discontinued LLC ATCC Cat#CL-101 L929 ATCC Cat#CRL-6364 THP-1 ATCC Cat#TIB-202 EMT6 ATCC Cat#CRL-2755 Lim1215 Laboratory of Prof. Chris Marshall N/A A431 ATCC Cat#CRL-1555 F3II Laboratory of Prof. Daniel Alonso N/A D2A1 Laboratory of Prof. Clare Isacke N/A Kym-1 Laboratory of Prof. John Silke N/A Immortalized MDFs Charles River (Cells generated in house) N/A MCA-205 Sigma Aldrich Cat#SCC-173 EO771 CH3 Biosystems Cat#94A001 MC38 Kerafast Cat#ENH204-FP Wildtype BMDMs and Lung fibroblasts (C57BL/6) Charles River (Cells generated in house) N/A Spata2-/- BMDMs (C57BL/6) Laboratory of Prof. Mads Gyrd-Hansen’s N/A Trif-/- BMDMs (C57BL/6) (Ticam1-/-) Laboratory of Prof. Manolis Pasparakis N/A Zbp1-/- BMDMs (C57BL/6) Laboratory of Prof. Manolis Pasparakis N/A Trif-/-Zbp1-/- BMDMs and LFs (C57BL/6) (Ticam1-/-Zbp1-/-) Laboratory of Prof. Manolis Pasparakis N/A L929 Tradd-/- This Manuscript N/A L929 Tradd-/- reconstituted with TRADDWT This Manuscript N/A L929 Tradd-/- reconstituted with TRADD DD-only This Manuscript N/A L929 Tradd-/- reconstituted with TRADD Y16A/F18A This Manuscript N/A (Continued on next page) e5 Immunity 57, 1–19.e1–e15, July 9, 2024

Sequencing:

Article Title: A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NaveniFlex MR Bethyl Laboratories Cat#NF.MR.100 CellTiter-GLO Promega Cat#G9681 RNeasy RNA extraction kit Qiagen Cat#74106 QuantiTec Reverse Transcription kit Qiagen Cat#205314 Pierce BCA protein assay kit Thermo Fisher Scientific Cat#23227 Deposited data Bulk RNA-seq (Sham Vs RT-treated EO771 cells) Sequence read archive ID: PRJNA1094427 Mass spectrometry: Human and mouse whole proteome analysis PRIDE ID: PXD043560 TCGA Pan-cancer analyses cBioPortal N/A SCAN-B TNBC patient data GEO Database GEO: GSE96058 GSEA analyses (hallmark gene set collection) The Molecular Signatures Database (MSigDB) N/A Raw western blots and image from this paper Mendeley https://doi.org/10.17632/822syk52yw.1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 HT1080 ATCC Cat#CCL-121 U937 ATCC Cat#CRL-3253 HT29 ATCC Cat#HTB-38 MDA-MB-231 ATCC Cat#CRM-HTB-26 MDA-MB-361 ATCC Cat#HTB-27 MDA-MB-468 ATCC Cat#HTB-132 HaCaT ATCC discontinued LLC ATCC Cat#CL-101 L929 ATCC Cat#CRL-6364 THP-1 ATCC Cat#TIB-202 EMT6 ATCC Cat#CRL-2755 Lim1215 Laboratory of Prof. Chris Marshall N/A A431 ATCC Cat#CRL-1555 F3II Laboratory of Prof. Daniel Alonso N/A D2A1 Laboratory of Prof. Clare Isacke N/A Kym-1 Laboratory of Prof. John Silke N/A Immortalized MDFs Charles River (Cells generated in house) N/A MCA-205 Sigma Aldrich Cat#SCC-173 EO771 CH3 Biosystems Cat#94A001 MC38 Kerafast Cat#ENH204-FP Wildtype BMDMs and Lung fibroblasts (C57BL/6) Charles River (Cells generated in house) N/A Spata2-/- BMDMs (C57BL/6) Laboratory of Prof. Mads Gyrd-Hansen’s N/A Trif-/- BMDMs (C57BL/6) (Ticam1-/-) Laboratory of Prof. Manolis Pasparakis N/A Zbp1-/- BMDMs (C57BL/6) Laboratory of Prof. Manolis Pasparakis N/A Trif-/-Zbp1-/- BMDMs and LFs (C57BL/6) (Ticam1-/-Zbp1-/-) Laboratory of Prof. Manolis Pasparakis N/A L929 Tradd-/- This Manuscript N/A L929 Tradd-/- reconstituted with TRADDWT This Manuscript N/A L929 Tradd-/- reconstituted with TRADD DD-only This Manuscript N/A L929 Tradd-/- reconstituted with TRADD Y16A/F18A This Manuscript N/A (Continued on next page) e5 Immunity 57, 1–19.e1–e15, July 9, 2024

Mass Spectrometry:

Article Title: A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NaveniFlex MR Bethyl Laboratories Cat#NF.MR.100 CellTiter-GLO Promega Cat#G9681 RNeasy RNA extraction kit Qiagen Cat#74106 QuantiTec Reverse Transcription kit Qiagen Cat#205314 Pierce BCA protein assay kit Thermo Fisher Scientific Cat#23227 Deposited data Bulk RNA-seq (Sham Vs RT-treated EO771 cells) Sequence read archive ID: PRJNA1094427 Mass spectrometry: Human and mouse whole proteome analysis PRIDE ID: PXD043560 TCGA Pan-cancer analyses cBioPortal N/A SCAN-B TNBC patient data GEO Database GEO: GSE96058 GSEA analyses (hallmark gene set collection) The Molecular Signatures Database (MSigDB) N/A Raw western blots and image from this paper Mendeley https://doi.org/10.17632/822syk52yw.1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 HT1080 ATCC Cat#CCL-121 U937 ATCC Cat#CRL-3253 HT29 ATCC Cat#HTB-38 MDA-MB-231 ATCC Cat#CRM-HTB-26 MDA-MB-361 ATCC Cat#HTB-27 MDA-MB-468 ATCC Cat#HTB-132 HaCaT ATCC discontinued LLC ATCC Cat#CL-101 L929 ATCC Cat#CRL-6364 THP-1 ATCC Cat#TIB-202 EMT6 ATCC Cat#CRL-2755 Lim1215 Laboratory of Prof. Chris Marshall N/A A431 ATCC Cat#CRL-1555 F3II Laboratory of Prof. Daniel Alonso N/A D2A1 Laboratory of Prof. Clare Isacke N/A Kym-1 Laboratory of Prof. John Silke N/A Immortalized MDFs Charles River (Cells generated in house) N/A MCA-205 Sigma Aldrich Cat#SCC-173 EO771 CH3 Biosystems Cat#94A001 MC38 Kerafast Cat#ENH204-FP Wildtype BMDMs and Lung fibroblasts (C57BL/6) Charles River (Cells generated in house) N/A Spata2-/- BMDMs (C57BL/6) Laboratory of Prof. Mads Gyrd-Hansen’s N/A Trif-/- BMDMs (C57BL/6) (Ticam1-/-) Laboratory of Prof. Manolis Pasparakis N/A Zbp1-/- BMDMs (C57BL/6) Laboratory of Prof. Manolis Pasparakis N/A Trif-/-Zbp1-/- BMDMs and LFs (C57BL/6) (Ticam1-/-Zbp1-/-) Laboratory of Prof. Manolis Pasparakis N/A L929 Tradd-/- This Manuscript N/A L929 Tradd-/- reconstituted with TRADDWT This Manuscript N/A L929 Tradd-/- reconstituted with TRADD DD-only This Manuscript N/A L929 Tradd-/- reconstituted with TRADD Y16A/F18A This Manuscript N/A (Continued on next page) e5 Immunity 57, 1–19.e1–e15, July 9, 2024

Western Blot:

Article Title: A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NaveniFlex MR Bethyl Laboratories Cat#NF.MR.100 CellTiter-GLO Promega Cat#G9681 RNeasy RNA extraction kit Qiagen Cat#74106 QuantiTec Reverse Transcription kit Qiagen Cat#205314 Pierce BCA protein assay kit Thermo Fisher Scientific Cat#23227 Deposited data Bulk RNA-seq (Sham Vs RT-treated EO771 cells) Sequence read archive ID: PRJNA1094427 Mass spectrometry: Human and mouse whole proteome analysis PRIDE ID: PXD043560 TCGA Pan-cancer analyses cBioPortal N/A SCAN-B TNBC patient data GEO Database GEO: GSE96058 GSEA analyses (hallmark gene set collection) The Molecular Signatures Database (MSigDB) N/A Raw western blots and image from this paper Mendeley https://doi.org/10.17632/822syk52yw.1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 HT1080 ATCC Cat#CCL-121 U937 ATCC Cat#CRL-3253 HT29 ATCC Cat#HTB-38 MDA-MB-231 ATCC Cat#CRM-HTB-26 MDA-MB-361 ATCC Cat#HTB-27 MDA-MB-468 ATCC Cat#HTB-132 HaCaT ATCC discontinued LLC ATCC Cat#CL-101 L929 ATCC Cat#CRL-6364 THP-1 ATCC Cat#TIB-202 EMT6 ATCC Cat#CRL-2755 Lim1215 Laboratory of Prof. Chris Marshall N/A A431 ATCC Cat#CRL-1555 F3II Laboratory of Prof. Daniel Alonso N/A D2A1 Laboratory of Prof. Clare Isacke N/A Kym-1 Laboratory of Prof. John Silke N/A Immortalized MDFs Charles River (Cells generated in house) N/A MCA-205 Sigma Aldrich Cat#SCC-173 EO771 CH3 Biosystems Cat#94A001 MC38 Kerafast Cat#ENH204-FP Wildtype BMDMs and Lung fibroblasts (C57BL/6) Charles River (Cells generated in house) N/A Spata2-/- BMDMs (C57BL/6) Laboratory of Prof. Mads Gyrd-Hansen’s N/A Trif-/- BMDMs (C57BL/6) (Ticam1-/-) Laboratory of Prof. Manolis Pasparakis N/A Zbp1-/- BMDMs (C57BL/6) Laboratory of Prof. Manolis Pasparakis N/A Trif-/-Zbp1-/- BMDMs and LFs (C57BL/6) (Ticam1-/-Zbp1-/-) Laboratory of Prof. Manolis Pasparakis N/A L929 Tradd-/- This Manuscript N/A L929 Tradd-/- reconstituted with TRADDWT This Manuscript N/A L929 Tradd-/- reconstituted with TRADD DD-only This Manuscript N/A L929 Tradd-/- reconstituted with TRADD Y16A/F18A This Manuscript N/A (Continued on next page) e5 Immunity 57, 1–19.e1–e15, July 9, 2024

Multiple Displacement Amplification:

Article Title: A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NaveniFlex MR Bethyl Laboratories Cat#NF.MR.100 CellTiter-GLO Promega Cat#G9681 RNeasy RNA extraction kit Qiagen Cat#74106 QuantiTec Reverse Transcription kit Qiagen Cat#205314 Pierce BCA protein assay kit Thermo Fisher Scientific Cat#23227 Deposited data Bulk RNA-seq (Sham Vs RT-treated EO771 cells) Sequence read archive ID: PRJNA1094427 Mass spectrometry: Human and mouse whole proteome analysis PRIDE ID: PXD043560 TCGA Pan-cancer analyses cBioPortal N/A SCAN-B TNBC patient data GEO Database GEO: GSE96058 GSEA analyses (hallmark gene set collection) The Molecular Signatures Database (MSigDB) N/A Raw western blots and image from this paper Mendeley https://doi.org/10.17632/822syk52yw.1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 HT1080 ATCC Cat#CCL-121 U937 ATCC Cat#CRL-3253 HT29 ATCC Cat#HTB-38 MDA-MB-231 ATCC Cat#CRM-HTB-26 MDA-MB-361 ATCC Cat#HTB-27 MDA-MB-468 ATCC Cat#HTB-132 HaCaT ATCC discontinued LLC ATCC Cat#CL-101 L929 ATCC Cat#CRL-6364 THP-1 ATCC Cat#TIB-202 EMT6 ATCC Cat#CRL-2755 Lim1215 Laboratory of Prof. Chris Marshall N/A A431 ATCC Cat#CRL-1555 F3II Laboratory of Prof. Daniel Alonso N/A D2A1 Laboratory of Prof. Clare Isacke N/A Kym-1 Laboratory of Prof. John Silke N/A Immortalized MDFs Charles River (Cells generated in house) N/A MCA-205 Sigma Aldrich Cat#SCC-173 EO771 CH3 Biosystems Cat#94A001 MC38 Kerafast Cat#ENH204-FP Wildtype BMDMs and Lung fibroblasts (C57BL/6) Charles River (Cells generated in house) N/A Spata2-/- BMDMs (C57BL/6) Laboratory of Prof. Mads Gyrd-Hansen’s N/A Trif-/- BMDMs (C57BL/6) (Ticam1-/-) Laboratory of Prof. Manolis Pasparakis N/A Zbp1-/- BMDMs (C57BL/6) Laboratory of Prof. Manolis Pasparakis N/A Trif-/-Zbp1-/- BMDMs and LFs (C57BL/6) (Ticam1-/-Zbp1-/-) Laboratory of Prof. Manolis Pasparakis N/A L929 Tradd-/- This Manuscript N/A L929 Tradd-/- reconstituted with TRADDWT This Manuscript N/A L929 Tradd-/- reconstituted with TRADD DD-only This Manuscript N/A L929 Tradd-/- reconstituted with TRADD Y16A/F18A This Manuscript N/A (Continued on next page) e5 Immunity 57, 1–19.e1–e15, July 9, 2024

Generated:

Article Title: A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NaveniFlex MR Bethyl Laboratories Cat#NF.MR.100 CellTiter-GLO Promega Cat#G9681 RNeasy RNA extraction kit Qiagen Cat#74106 QuantiTec Reverse Transcription kit Qiagen Cat#205314 Pierce BCA protein assay kit Thermo Fisher Scientific Cat#23227 Deposited data Bulk RNA-seq (Sham Vs RT-treated EO771 cells) Sequence read archive ID: PRJNA1094427 Mass spectrometry: Human and mouse whole proteome analysis PRIDE ID: PXD043560 TCGA Pan-cancer analyses cBioPortal N/A SCAN-B TNBC patient data GEO Database GEO: GSE96058 GSEA analyses (hallmark gene set collection) The Molecular Signatures Database (MSigDB) N/A Raw western blots and image from this paper Mendeley https://doi.org/10.17632/822syk52yw.1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 HT1080 ATCC Cat#CCL-121 U937 ATCC Cat#CRL-3253 HT29 ATCC Cat#HTB-38 MDA-MB-231 ATCC Cat#CRM-HTB-26 MDA-MB-361 ATCC Cat#HTB-27 MDA-MB-468 ATCC Cat#HTB-132 HaCaT ATCC discontinued LLC ATCC Cat#CL-101 L929 ATCC Cat#CRL-6364 THP-1 ATCC Cat#TIB-202 EMT6 ATCC Cat#CRL-2755 Lim1215 Laboratory of Prof. Chris Marshall N/A A431 ATCC Cat#CRL-1555 F3II Laboratory of Prof. Daniel Alonso N/A D2A1 Laboratory of Prof. Clare Isacke N/A Kym-1 Laboratory of Prof. John Silke N/A Immortalized MDFs Charles River (Cells generated in house) N/A MCA-205 Sigma Aldrich Cat#SCC-173 EO771 CH3 Biosystems Cat#94A001 MC38 Kerafast Cat#ENH204-FP Wildtype BMDMs and Lung fibroblasts (C57BL/6) Charles River (Cells generated in house) N/A Spata2-/- BMDMs (C57BL/6) Laboratory of Prof. Mads Gyrd-Hansen’s N/A Trif-/- BMDMs (C57BL/6) (Ticam1-/-) Laboratory of Prof. Manolis Pasparakis N/A Zbp1-/- BMDMs (C57BL/6) Laboratory of Prof. Manolis Pasparakis N/A Trif-/-Zbp1-/- BMDMs and LFs (C57BL/6) (Ticam1-/-Zbp1-/-) Laboratory of Prof. Manolis Pasparakis N/A L929 Tradd-/- This Manuscript N/A L929 Tradd-/- reconstituted with TRADDWT This Manuscript N/A L929 Tradd-/- reconstituted with TRADD DD-only This Manuscript N/A L929 Tradd-/- reconstituted with TRADD Y16A/F18A This Manuscript N/A (Continued on next page) e5 Immunity 57, 1–19.e1–e15, July 9, 2024

Incubation:

Article Title: A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NaveniFlex MR Bethyl Laboratories Cat#NF.MR.100 CellTiter-GLO Promega Cat#G9681 RNeasy RNA extraction kit Qiagen Cat#74106 QuantiTec Reverse Transcription kit Qiagen Cat#205314 Pierce BCA protein assay kit Thermo Fisher Scientific Cat#23227 Deposited data Bulk RNA-seq (Sham Vs RT-treated EO771 cells) Sequence read archive ID: PRJNA1094427 Mass spectrometry: Human and mouse whole proteome analysis PRIDE ID: PXD043560 TCGA Pan-cancer analyses cBioPortal N/A SCAN-B TNBC patient data GEO Database GEO: GSE96058 GSEA analyses (hallmark gene set collection) The Molecular Signatures Database (MSigDB) N/A Raw western blots and image from this paper Mendeley https://doi.org/10.17632/822syk52yw.1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 HT1080 ATCC Cat#CCL-121 U937 ATCC Cat#CRL-3253 HT29 ATCC Cat#HTB-38 MDA-MB-231 ATCC Cat#CRM-HTB-26 MDA-MB-361 ATCC Cat#HTB-27 MDA-MB-468 ATCC Cat#HTB-132 HaCaT ATCC discontinued LLC ATCC Cat#CL-101 L929 ATCC Cat#CRL-6364 THP-1 ATCC Cat#TIB-202 EMT6 ATCC Cat#CRL-2755 Lim1215 Laboratory of Prof. Chris Marshall N/A A431 ATCC Cat#CRL-1555 F3II Laboratory of Prof. Daniel Alonso N/A D2A1 Laboratory of Prof. Clare Isacke N/A Kym-1 Laboratory of Prof. John Silke N/A Immortalized MDFs Charles River (Cells generated in house) N/A MCA-205 Sigma Aldrich Cat#SCC-173 EO771 CH3 Biosystems Cat#94A001 MC38 Kerafast Cat#ENH204-FP Wildtype BMDMs and Lung fibroblasts (C57BL/6) Charles River (Cells generated in house) N/A Spata2-/- BMDMs (C57BL/6) Laboratory of Prof. Mads Gyrd-Hansen’s N/A Trif-/- BMDMs (C57BL/6) (Ticam1-/-) Laboratory of Prof. Manolis Pasparakis N/A Zbp1-/- BMDMs (C57BL/6) Laboratory of Prof. Manolis Pasparakis N/A Trif-/-Zbp1-/- BMDMs and LFs (C57BL/6) (Ticam1-/-Zbp1-/-) Laboratory of Prof. Manolis Pasparakis N/A L929 Tradd-/- This Manuscript N/A L929 Tradd-/- reconstituted with TRADDWT This Manuscript N/A L929 Tradd-/- reconstituted with TRADD DD-only This Manuscript N/A L929 Tradd-/- reconstituted with TRADD Y16A/F18A This Manuscript N/A (Continued on next page) e5 Immunity 57, 1–19.e1–e15, July 9, 2024

Expressing:

Article Title: A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NaveniFlex MR Bethyl Laboratories Cat#NF.MR.100 CellTiter-GLO Promega Cat#G9681 RNeasy RNA extraction kit Qiagen Cat#74106 QuantiTec Reverse Transcription kit Qiagen Cat#205314 Pierce BCA protein assay kit Thermo Fisher Scientific Cat#23227 Deposited data Bulk RNA-seq (Sham Vs RT-treated EO771 cells) Sequence read archive ID: PRJNA1094427 Mass spectrometry: Human and mouse whole proteome analysis PRIDE ID: PXD043560 TCGA Pan-cancer analyses cBioPortal N/A SCAN-B TNBC patient data GEO Database GEO: GSE96058 GSEA analyses (hallmark gene set collection) The Molecular Signatures Database (MSigDB) N/A Raw western blots and image from this paper Mendeley https://doi.org/10.17632/822syk52yw.1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 HT1080 ATCC Cat#CCL-121 U937 ATCC Cat#CRL-3253 HT29 ATCC Cat#HTB-38 MDA-MB-231 ATCC Cat#CRM-HTB-26 MDA-MB-361 ATCC Cat#HTB-27 MDA-MB-468 ATCC Cat#HTB-132 HaCaT ATCC discontinued LLC ATCC Cat#CL-101 L929 ATCC Cat#CRL-6364 THP-1 ATCC Cat#TIB-202 EMT6 ATCC Cat#CRL-2755 Lim1215 Laboratory of Prof. Chris Marshall N/A A431 ATCC Cat#CRL-1555 F3II Laboratory of Prof. Daniel Alonso N/A D2A1 Laboratory of Prof. Clare Isacke N/A Kym-1 Laboratory of Prof. John Silke N/A Immortalized MDFs Charles River (Cells generated in house) N/A MCA-205 Sigma Aldrich Cat#SCC-173 EO771 CH3 Biosystems Cat#94A001 MC38 Kerafast Cat#ENH204-FP Wildtype BMDMs and Lung fibroblasts (C57BL/6) Charles River (Cells generated in house) N/A Spata2-/- BMDMs (C57BL/6) Laboratory of Prof. Mads Gyrd-Hansen’s N/A Trif-/- BMDMs (C57BL/6) (Ticam1-/-) Laboratory of Prof. Manolis Pasparakis N/A Zbp1-/- BMDMs (C57BL/6) Laboratory of Prof. Manolis Pasparakis N/A Trif-/-Zbp1-/- BMDMs and LFs (C57BL/6) (Ticam1-/-Zbp1-/-) Laboratory of Prof. Manolis Pasparakis N/A L929 Tradd-/- This Manuscript N/A L929 Tradd-/- reconstituted with TRADDWT This Manuscript N/A L929 Tradd-/- reconstituted with TRADD DD-only This Manuscript N/A L929 Tradd-/- reconstituted with TRADD Y16A/F18A This Manuscript N/A (Continued on next page) e5 Immunity 57, 1–19.e1–e15, July 9, 2024

Comparison:

Article Title: A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NaveniFlex MR Bethyl Laboratories Cat#NF.MR.100 CellTiter-GLO Promega Cat#G9681 RNeasy RNA extraction kit Qiagen Cat#74106 QuantiTec Reverse Transcription kit Qiagen Cat#205314 Pierce BCA protein assay kit Thermo Fisher Scientific Cat#23227 Deposited data Bulk RNA-seq (Sham Vs RT-treated EO771 cells) Sequence read archive ID: PRJNA1094427 Mass spectrometry: Human and mouse whole proteome analysis PRIDE ID: PXD043560 TCGA Pan-cancer analyses cBioPortal N/A SCAN-B TNBC patient data GEO Database GEO: GSE96058 GSEA analyses (hallmark gene set collection) The Molecular Signatures Database (MSigDB) N/A Raw western blots and image from this paper Mendeley https://doi.org/10.17632/822syk52yw.1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 HT1080 ATCC Cat#CCL-121 U937 ATCC Cat#CRL-3253 HT29 ATCC Cat#HTB-38 MDA-MB-231 ATCC Cat#CRM-HTB-26 MDA-MB-361 ATCC Cat#HTB-27 MDA-MB-468 ATCC Cat#HTB-132 HaCaT ATCC discontinued LLC ATCC Cat#CL-101 L929 ATCC Cat#CRL-6364 THP-1 ATCC Cat#TIB-202 EMT6 ATCC Cat#CRL-2755 Lim1215 Laboratory of Prof. Chris Marshall N/A A431 ATCC Cat#CRL-1555 F3II Laboratory of Prof. Daniel Alonso N/A D2A1 Laboratory of Prof. Clare Isacke N/A Kym-1 Laboratory of Prof. John Silke N/A Immortalized MDFs Charles River (Cells generated in house) N/A MCA-205 Sigma Aldrich Cat#SCC-173 EO771 CH3 Biosystems Cat#94A001 MC38 Kerafast Cat#ENH204-FP Wildtype BMDMs and Lung fibroblasts (C57BL/6) Charles River (Cells generated in house) N/A Spata2-/- BMDMs (C57BL/6) Laboratory of Prof. Mads Gyrd-Hansen’s N/A Trif-/- BMDMs (C57BL/6) (Ticam1-/-) Laboratory of Prof. Manolis Pasparakis N/A Zbp1-/- BMDMs (C57BL/6) Laboratory of Prof. Manolis Pasparakis N/A Trif-/-Zbp1-/- BMDMs and LFs (C57BL/6) (Ticam1-/-Zbp1-/-) Laboratory of Prof. Manolis Pasparakis N/A L929 Tradd-/- This Manuscript N/A L929 Tradd-/- reconstituted with TRADDWT This Manuscript N/A L929 Tradd-/- reconstituted with TRADD DD-only This Manuscript N/A L929 Tradd-/- reconstituted with TRADD Y16A/F18A This Manuscript N/A (Continued on next page) e5 Immunity 57, 1–19.e1–e15, July 9, 2024

Activation Assay:

Article Title: A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NaveniFlex MR Bethyl Laboratories Cat#NF.MR.100 CellTiter-GLO Promega Cat#G9681 RNeasy RNA extraction kit Qiagen Cat#74106 QuantiTec Reverse Transcription kit Qiagen Cat#205314 Pierce BCA protein assay kit Thermo Fisher Scientific Cat#23227 Deposited data Bulk RNA-seq (Sham Vs RT-treated EO771 cells) Sequence read archive ID: PRJNA1094427 Mass spectrometry: Human and mouse whole proteome analysis PRIDE ID: PXD043560 TCGA Pan-cancer analyses cBioPortal N/A SCAN-B TNBC patient data GEO Database GEO: GSE96058 GSEA analyses (hallmark gene set collection) The Molecular Signatures Database (MSigDB) N/A Raw western blots and image from this paper Mendeley https://doi.org/10.17632/822syk52yw.1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 HT1080 ATCC Cat#CCL-121 U937 ATCC Cat#CRL-3253 HT29 ATCC Cat#HTB-38 MDA-MB-231 ATCC Cat#CRM-HTB-26 MDA-MB-361 ATCC Cat#HTB-27 MDA-MB-468 ATCC Cat#HTB-132 HaCaT ATCC discontinued LLC ATCC Cat#CL-101 L929 ATCC Cat#CRL-6364 THP-1 ATCC Cat#TIB-202 EMT6 ATCC Cat#CRL-2755 Lim1215 Laboratory of Prof. Chris Marshall N/A A431 ATCC Cat#CRL-1555 F3II Laboratory of Prof. Daniel Alonso N/A D2A1 Laboratory of Prof. Clare Isacke N/A Kym-1 Laboratory of Prof. John Silke N/A Immortalized MDFs Charles River (Cells generated in house) N/A MCA-205 Sigma Aldrich Cat#SCC-173 EO771 CH3 Biosystems Cat#94A001 MC38 Kerafast Cat#ENH204-FP Wildtype BMDMs and Lung fibroblasts (C57BL/6) Charles River (Cells generated in house) N/A Spata2-/- BMDMs (C57BL/6) Laboratory of Prof. Mads Gyrd-Hansen’s N/A Trif-/- BMDMs (C57BL/6) (Ticam1-/-) Laboratory of Prof. Manolis Pasparakis N/A Zbp1-/- BMDMs (C57BL/6) Laboratory of Prof. Manolis Pasparakis N/A Trif-/-Zbp1-/- BMDMs and LFs (C57BL/6) (Ticam1-/-Zbp1-/-) Laboratory of Prof. Manolis Pasparakis N/A L929 Tradd-/- This Manuscript N/A L929 Tradd-/- reconstituted with TRADDWT This Manuscript N/A L929 Tradd-/- reconstituted with TRADD DD-only This Manuscript N/A L929 Tradd-/- reconstituted with TRADD Y16A/F18A This Manuscript N/A (Continued on next page) e5 Immunity 57, 1–19.e1–e15, July 9, 2024

Review